Results 301-310 of about 359
- The Gene Ontology Consortium (GOC) produces a wealth of resources widely used throughout the scientific community. In this chapter, we discuss the different ways in which researchers can access the resources of the GOC. We here share details about
Date: (2017) Authors: Monica Munoz-Torres , Seth Carbon Ref: The Gene Ontology Handbook - Annotation of Alternatively Spliced Proteins and Transcripts with Protein-Folding Algorithms and Isoform-Level Functiona... new windowTens of thousands of splice isoforms of proteins have been catalogued as predicted sequences from transcripts in humans and other species. Relatively few have been characterized biochemically or structurally. With the extensive development of protein
Date: (2017) Authors: Hongdong Li , Yang Zhang , Yuanfang Guan , Rajasree Menon , Gilbert S. Omenn Ref: Protein Bioinformatics - By capturing and barcoding RNA in its native tissue location, researchers can visualize and quantify gene expression in situ.
Date: (2016) Authors: Nicole Rusk - Application of Systems Theory in Longitudinal Studies on the Origin and Progression of Alzheimer’s Disease new windowThis chapter questions the prevailing “implicit” assumption that molecular mechanisms and the biological phenotype of dominantly inherited early-onset alzheimer’s disease (EOAD) could serve as a linear model to study the pathogenesis of sporadic
Date: (2016) Authors: Simone Lista , Zaven S. Khachaturian , Dan Rujescu , Francesco Garaci ... Harald Hampel Ref: Systems Biology of Alzheimer's Disease - Microorganisms are most diverse and abundant life on Earth, harbouring novel metabolic pathways and synthesising potentially useful products. It is apparent that genetically identical microorganisms exposed to the same environmental conditions show
Date: (2016) Authors: Wei E. Huang Ref: Hydrocarbon and Lipid Microbiology Protocols - Protocols for Probing Genome Architecture of Regulatory Networks in Hydrocarbon and Lipid Microorganisms new windowGenome architecture and the regulation of gene expression are expected to be interdependent. Understanding this interdependence is key to successful genome engineering. Evidence for nonrandom arrangement of genes along genomes, defined as the
Date: (2016) Authors: Costas Bouyioukos , Mohamed Elati , François Képès Ref: Hydrocarbon and Lipid Microbiology Protocols - Genome-wide profiling of RNA polymerase transcription at nucleotide resolution in human cells with native elongating tra... new windowMany features of how gene transcription occurs in human cells remain unclear, mainly because of a lack of quantitative approaches to follow genome transcription with nucleotide precision in vivo. Here we present a robust genome-wide approach for
Date: (2016) Authors: Andreas Mayer , L Stirling Churchman - A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods
Date: (2016) Authors: Suguna Rani Krishnaswami , Rashel V Grindberg , Mark Novotny , Pratap Venepally ... Roger S Lasken - Cell-type-specific profiling of protein–DNA interactions without cell isolation using targeted DamID with next-generatio... new windowThis protocol is an extension to:Nat. Protoc.2, 1467–1478 (2007); doi:10.1038/nprot.2007.148; published online 7 June 2007 The ability to profile transcription and chromatin binding in a cell-type-specific manner is a
Date: (2016) Authors: Owen J Marshall , Tony D Southall , Seth W Cheetham , Andrea H Brand - Microarray and RNA-sequencing technology now exists for the characterization of the Ambystoma mexicanum transcriptome. With sufficient replication, these tools give the opportunity to truly investigate gene expression in a variety of experimental
Date: (2015) Authors: S. Randal Voss , Antony Athippozhy , M. Ryan Woodcock Ref: Salamanders in Regeneration Research