Results 401-410 of about 900
- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High-Confidence Genome-Wide Bindin... new windowChromatinimmunoprecipitation coupled to next generation sequencing (ChIP-seq) is a powerful tool to map context-dependent genome-wide binding of nuclear hormone receptors and their coregulators. This information can provide important mechanist
Date: (2019) Authors: Ashfaq Ali Mir , Kenneth Allen Dyar , Franziska Greulich , Fabiana Quagliarini ... Nina Henriette Uhlenhaut Ref: Nuclear Receptors - CLIP-Seq to identify targets and interactions of RNA binding proteins and RNA modifying enzymes. new window
Date: 2021-08-23 Authors: Stoute J, Liu KF. Journal: Methods in enzymology - Grosselin, K. et al. Nat. Genet. 51, 1060–1066 (2019). Rare cell types can have an outsized biological effect, but their sparsity makes them impossible to detect in bulk samples. To study the effect of such heterogeneity, Grosselin et al. developed a
Date: (2019) Authors: Nicole Rusk Date: 2021-08-21 Authors: Pantazi V, Berzsenyi I, Borsos BN, Pankotai T. Journal: Journal of visualized experiments : JoVE - Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroe... new windowThe acetylation of histone tails, which relaxes compact chromatin structure and enhances the accessibility of DNA to regulatory proteins, has emerged as a key mechanism for regulating gene expression. These modifications in turn play critical roles
Date: (2019) Authors: Craig Myrum , Peter R. Rapp Ref: Protein Acetylation - Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages. new window
Date: 2021-08-19 Authors: Tsuboi M, Gotoh Y. Journal: STAR protocols - Transcription coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ, also known as WWTR1) are homologs of the Drosophila Yorkie (Yki) protein and are major downstream effectors of the evolutionarily
Date: (2019) Authors: Xiaolei Cao , Bin Zhao Ref: The Hippo Pathway - Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag. new window
Date: 2021-08-19 Authors: Li Y, Nakka K, Olender T, Gingras-Gelinas P, Wong MM, Robinson DCL, Bandukwala H, Palii CG, Neyret O, Brand M, Blais A, Dilworth FJ. Journal: STAR protocols - A modified CUT&RUN protocol and analysis pipeline to identify transcription factor binding sites in human cell lines. new window
Date: 2021-08-17 Authors: Kong NR, Chai L, Tenen DG, Bassal MA. Journal: STAR protocols - Co-immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor α at Postsynaptic Density in Brain... new windowCo-immunoprecipitation is a traditional method but still one of the most powerful tools to identify novel interaction partners by increasing its specificity and decreasing false-positive result. In the current work, we show a robust procedure where
Date: (2019) Authors: Gen Murakami , Suguru Kawato Ref: Co-Immunoprecipitation Methods for Brain Tissue