Results 501-510 of about 900
- Chromatinimmunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick a
Date: (2018) Authors: Patrik Asp Ref: ChromatinImmunoprecipitation Date: 2021-01-01 Authors: Simon JM, Gomez NC. Journal: Methods in molecular biology (Clifton, N.J.) - A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis new windowChromatinimmunoprecipitation (ChIP) is a widely used and very powerful procedure to identify the proteins that are associated with the DNA to regulate developmental processes. These proteins can be transcription factors, or specific histone varian
Date: (2018) Authors: Bénédicte Desvoyes , Zaida Vergara , Joana Sequeira-Mendes , Sofia Madeira , Crisanto Gutierrez Ref: Plant Chromatin Dynamics - The development of novel high-throughput sequencing methods for ChIP (chromatinimmunoprecipitation) has provided a very powerful tool to study gene regulation in multiple conditions at unprecedented resolution and scale. Proactive quality-contr
Date: (2018) Authors: Ines Santiago , Thomas Carroll Ref: ChromatinImmunoprecipitation - Identification of chromatin modifications, e.g., histone acetylation and methylation, among others, is widely carried out by using a chromatinimmunoprecipitation (ChIP) strategy. The information obtained with these procedures is useful to gain
Date: (2018) Authors: Bénédicte Desvoyes , Joana Sequeira-Mendes , Zaida Vergara , Sofia Madeira , Crisanto Gutierrez Ref: Plant Chromatin Dynamics - Chromatinimmunoprecipitation is used to measure the binding of transcription factors to target DNA sequences in order to better understand transcriptional regulation. Here, we describe a process to analyze bacterial transcription factor binding
Date: (2018) Authors: Brett R. Hanson , Ming Tan Ref: ChromatinImmunoprecipitation Date: 2021-01-01 Authors: Fonseca Costa SS, Ripperger JA. Journal: Methods in molecular biology (Clifton, N.J.) - μChIP-Seq for Genome-Wide Mapping of In Vivo TF-DNA Interactions in Arabidopsis Root Protoplasts new windowChromatinimmunoprecipitation (ChIP) is a widely used method to map the position of DNA-binding proteins such as histones and transcription factors (TFs) upon their interaction with particular regions of the genome. To examine the genom
Date: (2018) Authors: Alessia Para , Ying Li , Gloria M. Coruzzi Ref: Root Development - Profiling Histone Modifications in Synchronized Floral Tissues for Quantitative Resolution of Chromatin and Transcriptom... new windowCovalent histone modifications and their effects on chromatin state and accessibility play a key role in the regulation of gene expression in eukaryotes. To gain insights into their functions during plant growth and development, the distribution of
Date: (2018) Authors: Julia Engelhorn , Frank Wellmer , Cristel C. Carles Ref: Plant Chromatin Dynamics - Chromatinimmunoprecipitation (ChIP) is the most widely used method to analyze protein–DNA interactions in vivo. Coupled with next generation sequencing, ChIP-seq experiments map protein–DNA interactions in a genome-wide fashion. Here we describe
Date: (2018) Authors: Chenhuan Xu , Victor G. Corces Ref: CpG Islands