Results 581-590 of about 900
- Corrigendum: Chromatin Immunoprecipitation for Chromatin Interaction Analysis Using Paired-End-Tag (ChIA-PET) Sequencing... new window
Date: 2020-01-02 Authors: Buisine N, Ruan X, Ruan Y, Sachs LM. Journal: Cold Spring Harbor protocols - Proximity-CLIP Provides a Snapshot of Protein-Occupied RNA Elements at Subcellular Resolution and Transcriptome-Wide Sca... new window
Date: 2020-01-01 Authors: Benhalevy D, Hafner M. Journal: Methods in molecular biology (Clifton, N.J.) - The ability to edit specific epigenetic modifications at defined gene loci is pivotal to understand the biological function of these epigenetic marks. Here we describe a new inducible method to integrate the dCas9-based genome targeting with abscisic
Date: (2018) Authors: Dan Gao , Fu-Sen Liang Ref: Epigenome Editing - Plant Individual Nucleotide Resolution Cross-Linking and Immunoprecipitation to Characterize RNA-Protein Complexes. new window
Date: 2020-01-01 Authors: Köster T, Staiger D. Journal: Methods in molecular biology (Clifton, N.J.) - Many experimental models have been used to study erythropoiesis. Even prior to the advent of the genetic manipulation of animal models, erythropoiesis was examined in the mouse, chicken, sheep, goat, and rabbit, among other vertebrates. Erythroid
Date: (2018) Authors: Joyce A. Lloyd Ref: Erythropoiesis - Mapping Transcriptome-Wide and Genome-Wide RNA-DNA Contacts with Chromatin-Associated RNA Sequencing (ChAR-seq). new window
Date: 2020-01-01 Authors: Limouse C, Jukam D, Smith OK, Fryer KA, Straight AF. Journal: Methods in molecular biology (Clifton, N.J.) - Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling new windowRegulation of gene expression is primarily controlled by changes in the proteins that occupy genes’ regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and
Date: (2018) Authors: Samuel A. Myers , Jason Wright , Ryan Peckner , Brian T. Kalish ... Steven A. Carr - Identification of Chromatin Binding Sites for Long Noncoding RNAs by Chromatin Oligo-Affinity Precipitation (ChOP). new window
Date: 2020-01-01 Authors: Rossi MN, Maione R. Journal: Methods in molecular biology (Clifton, N.J.) - Stereotaxic Surgery and Viral Delivery of Zinc-Finger Epigenetic Editing Tools in Rodent Brain new windowDelivery of engineered zinc-finger proteins (ZFPs) for targeted epigenetic remodeling in rodent brain can be facilitated by the use of viral vector-mediated gene transfer coupled with stereotaxic surgery techniques. Here we describe the surgical
Date: (2018) Authors: Peter J. Hamilton , Carissa J. Lim , Eric J. Nestler , Elizabeth A. Heller Ref: Zinc Finger Proteins - Genome editing with site-specific nucleases (SSNs) may be effective for gene therapy, as SSNs can modify target genes. However, the main limitation of genome editing for clinical use is off-target effects by excess amounts of SSNs within cells.
Date: (2018) Authors: Maui Nishio , Ayana Yamagishi , Kaori Tsukakoshi , Yoshio Kato ... Kazunori Ikebukuro Ref: Zinc Finger Proteins