Results 391-400 of about 900
- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High-Confidence Genome-Wide Bindin... new windowChromatinimmunoprecipitation coupled to next generation sequencing (ChIP-seq) is a powerful tool to map context-dependent genome-wide binding of nuclear hormone receptors and their coregulators. This information can provide important mechanist
Date: (2019) Authors: Ashfaq Ali Mir , Kenneth Allen Dyar , Franziska Greulich , Fabiana Quagliarini ... Nina Henriette Uhlenhaut Ref: Nuclear Receptors - CLIP-Seq to identify targets and interactions of RNA binding proteins and RNA modifying enzymes. new window
Date: 2021-08-23 Authors: Stoute J, Liu KF. Journal: Methods in enzymology - Grosselin, K. et al. Nat. Genet. 51, 1060–1066 (2019). Rare cell types can have an outsized biological effect, but their sparsity makes them impossible to detect in bulk samples. To study the effect of such heterogeneity, Grosselin et al. developed a
Date: (2019) Authors: Nicole Rusk Date: 2021-08-21 Authors: Pantazi V, Berzsenyi I, Borsos BN, Pankotai T. Journal: Journal of visualized experiments : JoVE - Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroe... new windowThe acetylation of histone tails, which relaxes compact chromatin structure and enhances the accessibility of DNA to regulatory proteins, has emerged as a key mechanism for regulating gene expression. These modifications in turn play critical roles
Date: (2019) Authors: Craig Myrum , Peter R. Rapp Ref: Protein Acetylation - Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages. new window
Date: 2021-08-19 Authors: Tsuboi M, Gotoh Y. Journal: STAR protocols - Transcription coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ, also known as WWTR1) are homologs of the Drosophila Yorkie (Yki) protein and are major downstream effectors of the evolutionarily
Date: (2019) Authors: Xiaolei Cao , Bin Zhao Ref: The Hippo Pathway - Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag. new window
Date: 2021-08-19 Authors: Li Y, Nakka K, Olender T, Gingras-Gelinas P, Wong MM, Robinson DCL, Bandukwala H, Palii CG, Neyret O, Brand M, Blais A, Dilworth FJ. Journal: STAR protocols - Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin
Date: (2019) Authors: Huilin Jin , Antonia Sophocleous , Asim Azfer , Stuart H. Ralston Ref: Bone Research Protocols - A modified CUT&RUN protocol and analysis pipeline to identify transcription factor binding sites in human cell lines. new window
Date: 2021-08-17 Authors: Kong NR, Chai L, Tenen DG, Bassal MA. Journal: STAR protocols