Results 451-460 of about 900
Date: 2021-01-22 Authors: Lau X, Yong PJA, Lou CKL, Munusamy P, Sangrithi M. Journal: STAR protocols - ChromatinImmunoprecipitation Assay in the Hyperthermoacidophilic Crenarchaeon, Sulfolobus acidocaldarius new windowChromatinimmunoprecipitation (ChIP) is a powerful method used for identifying genome-wide DNA–protein interactions in vivo. A large number of essential intracellular processes such as DNA replication, transcription regulation, chromatin stabilit
Date: (2018) Authors: Kun Wang , Ann-Christin Lindås Ref: ChromatinImmunoprecipitation - LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel lncRNA-Associated Proteins. new window
Date: 2021-01-01 Authors: Xing Z, Egranov S, Lin C, Yang L. Journal: Methods in molecular biology (Clifton, N.J.) - ChromatinImmunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angus... new windowChromatin is a dynamic entity that regulates different biological processes crucial for the proper functioning of the cell. Chromatin regulation depends largely on the interactions that occur between DNA with histones and nonhistone proteins. The
Date: (2018) Authors: Rosa Us-Camas , Clelia De-la-Peña Ref: Plant Cell Culture Protocols - Chromatinimmunoprecipitation (ChIP) allows determination of the locations to which a select protein is bound in chromatin. Chemical crosslinking of DNA and protein with bi-functional reagents such as formaldehyde and precipitation of the prote
Date: (2018) Authors: Ivan Krivega , Ann Dean Ref: Erythropoiesis - Chromatinimmunoprecipitation (ChIP) exploits the specific interactions between DNA and DNA-associated proteins. It can be used to examine a wide range of experimental parameters. A number of proteins bound at the same genomic location can identify
Date: (2018) Authors: Timothy V. Beischlag , Gratien G. Prefontaine , Oliver Hankinson Ref: ChromatinImmunoprecipitation - Chromatinimmunoprecipitation (ChIP), originally developed by John T. Lis and David Gilmour in 1984, has been useful to detect DNA sequences where protein(s) of interest bind. ChIP is comprised of several steps: (1) cross-linkin
Date: (2018) Authors: Miyoung Lee , Lorraine J. Gudas , Harold I. Saavedra Ref: The Retinoblastoma Protein - Analysis of Protein–DNA Interaction by ChromatinImmunoprecipitation and DNA Tiling Microarray (ChIP-on-chip) new windowChromatinimmunoprecipitation (ChIP) has become the most effective and widely used tool to study the interactions between specific proteins or modified forms of proteins and a genomic DNA region. Combined with genome-wide profiling technologies, su
Date: (2018) Authors: Hui Gao , Chunyan Zhao Ref: ChromatinImmunoprecipitation - MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale ChromatinImmunoprecipitation new windowIn multicellular organisms, the initiation and maintenance of specific cell types often require the activity of cell type-specific transcriptional regulators. Understanding their roles in gene regulation is crucial but probing their DNA targets in
Date: (2018) Authors: Shenqi Wang , On Sun Lau Ref: ChromatinImmunoprecipitation - Chromatinimmunoprecipitation (ChIP) is an invaluable method for studying interactions between histone proteins and genomic DNA regions and transcriptional regulation using antibodies to enrich genomic regions associated with these epitopes. Eith
Date: (2018) Authors: Amarjit Saini , Carl Johan Sundberg Ref: ChromatinImmunoprecipitation