Results 461-470 of about 900
- ChromatinImmunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next-Generation Seque... new windowChromatinimmunoprecipitation (ChIP) is considered the method of choice for characterizing interactions between a protein of interest and specific genomic regions. It is of paramount importance in gene-regulation studies, as it can be used to map t
Date: (2018) Authors: Mário A. Soares , Diogo S. Castro Ref: ChromatinImmunoprecipitation Date: 2021-01-01 Authors: Li S, Yan H, Lee J. Journal: Methods in molecular biology (Clifton, N.J.) - ChromatinImmunoprecipitation Sequencing (ChIP-Seq) for Transcription Factors and Chromatin Factors in Arabidopsis thalia... new windowChromatinimmunoprecipitation combined with next-generation sequencing (ChIP-seq) is a powerful technique to investigate in vivo transcription factor (TF) binding to DNA, as well as chromatin marks. Here we provide a detailed protocol for all the k
Date: (2018) Authors: Sandra Cortijo , Varodom Charoensawan , François Roudier , Philip A. Wigge Ref: Root Development - Epigenetic complexes regulate chromatin dynamics via binding to and assembling on chromatin. However, the mechanisms of chromatin binding and assembly of epigenetic complexes within cells remain incompletely understood, partly due to technical
Date: (2018) Authors: Roubina Tatavosian , Xiaojun Ren Ref: ChromatinImmunoprecipitation - Considerations on Experimental Design and Data Analysis of ChromatinImmunoprecipitation Experiments new windowArguably one of the most valuable techniques to study chromatin organization, ChIP is the method of choice to map the contacts established between proteins and genomic DNA. Ever since its inception, more than 30 years ago, ChIP has been constantly
Date: (2018) Authors: Antonio Jordán-Pla , Neus Visa Ref: ChromatinImmunoprecipitation Date: 2021-01-01 Authors: Jagatia H, Cantillon D. Journal: Methods in molecular biology (Clifton, N.J.) - ChromatinImmunoprecipitation and High-Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protoco... new windowChromatinimmunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become an essential tool for epigenetic scientists. ChIP-seq is used to map protein-DNA interactions and epigenetic marks such as histone modifications at t
Date: (2018) Authors: Darren K. Patten , Giacomo Corleone , Luca Magnani Ref: Epigenome Editing Date: 2021-01-01 Authors: Lister NC, Johnsson P, Waters PD, Morris KV. Journal: Methods in molecular biology (Clifton, N.J.) - Epigenetic regulation is achieved at many levels by different factors such as tissue-specific transcription factors, members of the basal transcriptional apparatus, chromatin-binding proteins, and noncoding RNAs. Importantly, chromatin structure
Date: (2018) Authors: Estela G. García-González , Bladimir Roque-Ramirez , Carlos Palma-Flores , J. Manuel Hernández-Hernández Ref: Mouse Embryogenesis - ChromatinImmunoprecipitation for Identification of Protein–DNA Interactions in Human Cells new windowChromatinimmunoprecipitation (ChIP) is a powerful technique allowing for investigation of protein–DNA interactions in living cells. Here, we provide a detailed step-by-step protocol for ChIP and highlight important considerations, challenges a
Date: (2018) Authors: Bjørk Ditlev Larsen , Martin Rønn Madsen , Ronni Nielsen , Susanne Mandrup Ref: Two-Hybrid Systems