Results 661-670 of about 900
- Over the past decade, multiple effector T cell subsets have been identified with varying differentiation conditions in the milieu as well as a broad diversity of cytokine expression. Interleukin-9 (IL-9) secreting T helper 9 (Th9) cells are the
Date: (2017) Authors: William Orent , Wassim Elyaman Ref: Th9 Cells Date: 2019-01-01 Authors: Yang D. Journal: Methods in molecular biology (Clifton, N.J.) - Resolving the DNA-binding specificities of transcription factors (TFs) is of critical value for understanding gene regulation. Here, we present a novel, semiautomated protein–DNA interaction characterization technology, selective microfluidics-based
Date: (2017) Authors: Alina Isakova , Romain Groux , Michael Imbeault , Pernille Rainer ... Bart Deplancke - Promoter-associated RNAs (paRNA) are long noncoding RNAs transcribed in sense or antisense direction in correspondence of promoters of other genes. Their expression influences promoter activity by means of specific regulatory function. PaRNA can act
Date: (2017) Authors: Sara Napoli Ref: Promoter Associated RNA - Applying the INTACT method to purify endosperm nuclei and to generate parental-specific epigenome profiles new windowThe early endosperm tissue of dicot species is very difficult to isolate by manual dissection. This protocol details how to apply the INTACT (isolation of nuclei tagged in specific cell types) system for isolating early endosperm nuclei of
Date: (2017) Authors: Jordi Moreno-Romero , Juan Santos-González , Lars Hennig , Claudia Köhler - Silenced heterochromatin influences all nuclear processes including chromosome structure, nuclear organization, transcription, replication, and repair. Proteins that mediate silencing affect all of these nuclear processes. Similarly proteins involved
Date: (2017) Authors: Misty R. Peterson , Omar Hamdani , Rohinton T. Kamakaka Ref: Cohesin and Condensin - Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein Binding in S. pombe new windowThe fission yeast Schizosaccharomyces pombe is especially well suited for studying meiosis in molecular detail. Experiments with S. pombe strains that undergo a nearly synchronous meiosis—at variable temperatures—have elucidated the mechanisms of
Date: (2017) Authors: Randy W. Hyppa , Kyle R. Fowler , Gerald R. Smith Ref: Meiosis - Isolation of pools of spermatogenic cells at specific developmental stages is essential for the investigations of molecular events controlling critical transitions during spermatogenesis. Large-scale cell purification techniques allow for combined
Date: (2017) Authors: Thierry Buchou , Minjia Tan , Sophie Barral , Anne-Laure Vitte ... Saadi Khochbin Ref: HDAC/HAT Function Assessment and Inhibitor Development - An Analysis of Pathological Activities of CCN Proteins in Joint Disorders: Mechanical Stretch-Mediated CCN2 Expression i... new windowThe multifunctional growth factor CYR61/CTGF/NOV (CCN) 2, also known as connective tissue growth factor, regulates cellular proliferation, differentiation, and tissue regeneration. Recent literatures have described important roles of CCN2 in the
Date: (2017) Authors: Takayuki Furumatsu , Toshifumi Ozaki Ref: CCN Proteins - Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry. new window
Date: 2019-01-01 Authors: Agudelo Garcia PA, Gardner M, Freitas MA, Parthun MR. Journal: Methods in molecular biology (Clifton, N.J.)