Results 471-480 of about 900
Date: 2021-01-01 Authors: Schoeftner S, Scarola M, Benetti R. Journal: Methods in molecular biology (Clifton, N.J.) - Chromatinimmunoprecipitation (ChIP) is becoming the standard method to study genome-wide distribution of histone variants and histone posttranslational modifications (PTMs). In this chapter, we describe a detailed native ChIP protocol and downstre
Date: (2018) Authors: Alicia Alonso , Emily Bernstein , Dan Hasson Ref: Histone Variants Date: 2021-01-01 Authors: Tang HY, Goldman AR, Zhang X, Speicher DW, Dang CV. Journal: Methods in molecular biology (Clifton, N.J.) - The Use of the ChromatinImmunoprecipitation Technique for In Vivo Identification of Plant Protein–DNA Interactions new windowTwo-hybrid systems allow for the identification of proteins that physically interact in the context of biological processes. In the cases where these proteins interact with DNA it is essential to define their binding properties with specific regions
Date: (2018) Authors: José A. Jarillo , Dorota N. Komar , Manuel Piñeiro Ref: Two-Hybrid Systems - Chromatinimmunoprecipitation (ChIP) is an invaluable method to characterize interactions between proteins and genomic DNA, such as the genomic localization of transcription factors and posttranslational modification of histones. DNA and proteins a
Date: (2018) Authors: Jessica B. Lee , Albert J. Keung Ref: Epigenome Editing - Analysis of Histone Modifications in Acute Myeloid Leukaemia Using ChromatinImmunoprecipitation new windowChromatinImmunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analys
Date: (2018) Authors: Benjamin J. Shields , Andrew Keniry , Marnie E. Blewitt , Matthew P. McCormack Ref: Inflammation and Cancer Date: 2021-01-01 Authors: Cameron DP, Kuzin V, Baranello L. Journal: Methods in molecular biology (Clifton, N.J.) - Chromatinimmunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein acro
Date: (2018) Authors: Julio C. Ayala , Jorge A. Benitez , Anisia J. Silva Ref: Vibrio Cholerae - ChromatinImmunoprecipitation of HIF-α in Breast Tumor Cells Using Wild Type and Loss of Function Models new windowChromatinimmunoprecipitation (ChIP) is a powerful method to determine whether a protein of interest binds to specific regulatory elements of the genome. Herein, we outline protocols optimized to detect binding of Hypoxia-Inducible Factor (HIF)-1α
Date: (2018) Authors: Danielle L. Brooks , Tiffany N. Seagroves Ref: Hypoxia Date: 2021-01-01 Authors: Rasmussen KD, Helin K. Journal: Methods in molecular biology (Clifton, N.J.)