Results 501-510 of about 900
- Chromatinimmunoprecipitation (ChIP) is the most widely used method to analyze protein–DNA interactions in vivo. Coupled with next generation sequencing, ChIP-seq experiments map protein–DNA interactions in a genome-wide fashion. Here we describe
Date: (2018) Authors: Chenhuan Xu , Victor G. Corces Ref: CpG Islands Date: 2021-01-01 Authors: Hill MC, Martin JF. Journal: Methods in molecular biology (Clifton, N.J.) - How to Tackle Challenging ChIP-Seq, with Long-Range Cross-Linking, Using ATRX as an Example new windowChromatinimmunoprecipitation coupled with high-throughput, next-generation DNA sequencing (ChIP-seq) has enabled researchers to establish the genome-wide patterns of chromatin modifications and binding of chromatin-associated protein
Date: (2018) Authors: Julia Truch , Jelena Telenius , Douglas R. Higgs , Richard J. Gibbons Ref: Histone Variants - Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants new windowAs a functional compartment, the nuclear periphery plays critical roles in regulating the spatial organization of chromatin in the nuclei and gene expression. Generally, the nuclear periphery is a transcriptionally repressed compartment enriched for
Date: (2018) Authors: Xiuli Bi , Chang Liu Ref: Plant Transcription Factors - Identifying Genomic Sites of ADP-Ribosylation Mediated by Specific Nuclear PARP Enzymes Using Click-ChIP new windowNuclear poly(ADP-ribose) polymerases (PARPs), including PARPs 1, 2, and 3 and the Tankyrases, belong to a family of enzymes that can bind to chromatin and covalently modify histone- and chromatin-associated proteins with ADP-ribose derived from
Date: (2018) Authors: Ryan A. Rogge , Bryan A. Gibson , W. Lee Kraus Ref: ADP-ribosylation and NAD+ Utilizing Enzymes Date: 2021-01-01 Authors: Yamaguchi M, Haber JE. Journal: Methods in molecular biology (Clifton, N.J.) - Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-seq) new windowMNase-seq allows the genome-wide examination of the nucleosome landscape by determination of nucleosome positioning and occupancy. Typically, native or formaldehyde fixed chromatin is subjected to digestion by micrococcal nuclease (MNase), which
Date: (2018) Authors: Wieteke Anna Hoeijmakers , Richárd Bártfai Ref: ChromatinImmunoprecipitation Date: 2021-01-01 Authors: Ma G, Babarinde IA, Zhuang Q, Hutchins AP. Journal: Methods in molecular biology (Clifton, N.J.) - An Integrated and Semiautomated Microscaled Approach to Profile Cis-Regulatory Elements by Histone Modification ChIP-Seq... new windowChromatinimmunoprecipitation followed by sequencing (ChIP-Seq) is the preferred approach to map histone modifications and identify cis-regulatory DNA elements throughout the genome. Multiple methods have been described to increase the efficiency o
Date: (2018) Authors: Diana Youhanna Jankeel , Justin Cayford , Benjamin Joachim Schmiedel , Pandurangan Vijayanand , Grégory Seumois Ref: Type 2 Immunity Date: 2021-01-01 Authors: Abakir A, Alenezi F, Ruzov A. Journal: Methods in molecular biology (Clifton, N.J.)