Mammalian meiosis is a highly specialized cell division process, resulting in the production of genetically unique haploid cells. However, the molecular mechanisms governing meiosis remain largely unknown, primarily due to the difficulty in isolating

The covalently closed circular DNA (cccDNA) of the hepatitis B virus (HBV) is organized as a minichromosome structure in the nucleus of infected hepatocytes and considered the major obstacle to the discovery of a cure for HBV. Until now, no

In plants, gene expression is orchestrated by thousands of transcription factors (TFs). For instance, a large set of bHLH TFs are involved in the regulation of iron homeostasis in Arabidopsis thaliana. The identification of the direct target genes of

Chromatinimmunoprecipitation followed by sequencing (ChIP-seq) is a widely used technique for genome-wide mapping of protein–DNA interactions and epigenetic marks in vivo. Recent studies have suggested an important role of heat sh

DNA-binding proteins and epigenetic modifications coordinate to regulate the spatiotemporal pattern of gene expression during development, and thus, profiling their genomic distribution on a genome-wide scale is of crucial significance for decoding

Chromatinimmunoprecipitation (ChIP) is one of the most widely used methods for investigating interactions between proteins and DNA sequences. ChIP plays an important role in the transcriptional regulation study, which can locate the target genes o

Chromatinimmunoprecipitation followed by high-throughput sequencing (ChIP-seq) is the most widely used method for analyzing genome-wide DNA–protein interactions. Because there is considerable variation in the modes and strengths of DNA–prote

Chromatinimmunoprecipitation (ChIP) is a widely used method to map protein–DNA interactions in vivo. Formaldehyde cross-linked chromatin is fragmented, and the protein of interest is immunoprecipitated using a specific antibody. T