Results 251-260 of about 900
- In vivo , genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolu... new window
Date: 2022-11-01 Authors: Tang JLY, Krautz R, Llorà-Batlle O, Hakes AE, Fox PM, Brand AH. Journal: STAR protocols - Genome-wide and sister chromatid-resolved profiling of protein occupancy in replicated chromatin with ChOR-seq and SCAR-... new windowElucidating the mechanisms underlying chromatin maintenance upon genome replication is critical for the understanding of how gene expression programs and cell identity are preserved across cell divisions. Here, we describe two recently developed
Date: (2021) Authors: Nataliya Petryk , Nazaret Reverón-Gómez , Cristina González-Aguilera , Maria Dalby ... Anja Groth - Parthenolide alleviates peritoneal fibrosis by inhibiting inflammation via the NF-κB/ TGF-β/Smad signaling axis. new window
Date: 2022-10-28 Authors: Zhang Y, Feng W, Peng X, Zhu L, Wang Z, Shen H, Chen C, Xiao L, Li S, Zhao Y, Lin M, Huang Y, Long H, Liang J. Journal: Laboratory investigation; a journal of technical methods and pathology - Functionalization of the genome is carried out by proteins that bind to DNA to regulate gene expression. Since this process is highly dynamic, context-dependent, and rarely performed by single proteins alone, we here describe ChIP-SICAP to identify
Date: (2021) Authors: Mahmoud-Reza Rafiee , Jeroen Krijgsveld Ref: Enhancers and Promoters - Multiomics data integration to reveal chromatin remodeling and reorganization induced by gene mutational synergy. new window
Date: 2022-10-14 Authors: Yun H, Vohra S, Lara-Astiaso D, Huntly BJP. Journal: STAR protocols - Chromatinimmunoprecipitation followed by sequencing (ChIP-seq) is a powerful tool to identify binding profiles of transcriptional regulators and chromatin regulators as well as histone modification patterns in a genome-wide manner. ChIP-seq consis
Date: (2021) Authors: Akira Yamakawa , Hironori Hojo , Shinsuke Ohba Ref: Chondrocytes Date: 2022-09-29 Authors: Khosraviani N, Abraham KJ, Chan JNY, Mekhail K. Journal: STAR protocols - Genome-Wide Profiling of Protein–DNA Interactions with Chromatin Endogenous Cleavage and High-Throughput Sequencing (ChE... new windowInteractions between regulatory proteins and specific genomic regions are critical for all chromatin-based processes, including transcription, DNA replication, and DNA repair. Genome-wide mapping of such interactions is most commonly performed with
Date: (2021) Authors: Moustafa M. Saleh , Jason P. Tourigny , Gabriel E. Zentner Ref: Enhancers and Promoters - Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevis... new window
Date: 2022-09-11 Authors: Reitz D, Savocco J, Piazza A, Heyer WD. Journal: Journal of visualized experiments : JoVE - Use of ChIP-qPCR to Study the Crosstalk Between HIF and NF-κB Signaling in Hypoxia and Normoxia new windowHypoxia and inflammation are intensely connected in a functional crosstalk. Within this crosstalk, two major transcription factors take center stage: HIF and NF-κB. To investigate transcription factor function, an important aspect is its ability to
Date: (2021) Authors: Dilem Shakir , Michael Batie , Sonia Rocha Ref: NF-κB Transcription Factors