Results 71-80 of about 900
- Protocol to target a promoter region in human embryonic kidney cells using the CRISPR-dCas9 system for single-locus prot... new window
Date: 2024-04-30 Authors: Alkhayer R, Ponath V, Pogge von Strandmann E. Journal: STAR protocols - Prediction of cell-type-specific cohesin-mediated chromatin loops based on chromatin state. new window
Date: 2024-04-24 Authors: Liu L, Jia R, Hou R, Huang C. Journal: Methods (San Diego, Calif.) - Enhancing Cell Line Stability by CRISPR/Cas9-Mediated Site-Specific Integration Based on Histone Modifications new windowIn traditional cell line design pipelines, cost- and time-intensive long-term stability studies must be performed due to random integration of the transgene into the genome. By this, integration into epigenetically silenced regions can lead to
Date: (2024) Authors: Oliver Hertel , Anne Neuss Ref: Recombinant Protein Expression in Mammalian Cells - Predictive and Prognostic Biomarkers in Patients With Mycosis Fungoides and Sézary Syndrome (BIO-MUSE): Protocol for a T... new window
Date: 2024-04-04 Authors: Belfrage E, Ek S, Johansson Å, Brauner H, Sonesson A, Drott K. Journal: JMIR research protocols - Mapping Meiotic DNA Breaks: Two Fully-Automated Pipelines to Analyze Single-Strand DNA Sequencing Data, hotSSDS and hotS... new windowMolecular approaches are required to detect DNA double-strand break (DSB) events and to map and quantify them at high resolution. One of the most popular molecular methods in the field of meiotic recombination is the ChIP-SSDS (Chromatin
Date: (2024) Authors: Pauline Auffret , Bernard Massy , Julie A. Clément Ref: Germ Cell Development - Protocol for generating customizable and reproducible plots of sequencing coverage data using the seqNdisplayR package. new window
Date: 2024-03-18 Authors: Lykke-Andersen S, Rouvière JO, Schmid M, Gockert M, Jensen TH. Journal: STAR protocols - Targeted Modification of Epigenetic Marks Using CRISPR/dCas9-SunTag-Based Modular Epigenetic Toolkit new windowThe epigenome, consisting of chemical modifications to DNA and histone proteins, can alter gene expression. Clustered regularly interspaced short palindromic repeats/dead CRISPR-associated protein 9 (CRISPR/dCas9) systems enable precise target
Date: (2024) Authors: Min Kyung Song , Yoon-Seong Kim Ref: Neuroprotection - Using synthetic genome readers/regulators to interrogate chromatin processes: A brief review. new window
Date: 2024-03-11 Authors: Philips SJ, Danda A, Ansari AZ. Journal: Methods (San Diego, Calif.) - SPO-Seq: An Accessible Method for Efficient Evaluation of Spo11 Catalytic Activity and Profiling Meiotic DSB Hotspots in... new windowMeiotic recombination is a key process facilitating the formation of crossovers and the exchange of genetic material between homologous chromosomes in early meiosis. This involves controlled double-strand breaks (DSBs) formation catalyzed by Spo11.
Date: (2024) Authors: Iván Hernández , David Álvarez-Melo , Mario García Lacoba , Jesús A. Carballo Ref: Meiosis - Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research. new window
Date: 2024-03-01 Authors: Li Y, Wu X, Hu P. Journal: Journal of visualized experiments : JoVE