Results 241-250 of about 900
- ChromatinImmunoprecipitation Followed by Next-Generation Sequencing (ChIP-Seq) Analysis in Ewing Sarcoma new windowChIP-seq is the method of choice for profiling protein–DNA interactions, and notably for characterizing the landscape of transcription factor binding and histone modifications. This technique has been widely used to study numerous aspects of tumor
Date: (2021) Authors: Gwenneg Kerdivel , Valentina Boeva Ref: Ewing Sarcoma - A High-Throughput ChromatinImmunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape new windowMYC is a transcription factor playing multiple functions both in physiological and pathological settings. Biochemical characterizations, combined with the analyses of MYC chromatin binding, have shown that its pleiotropic activity depends on the
Date: (2021) Authors: Luca Fagnocchi , Alessio Zippo Ref: The Myc Gene - Quantification of protein enrichment at site-specific DNA double-strand breaks by chromatin immunoprecipitation in cultu... new window
Date: 2022-12-14 Authors: Sharma AK, Fitieh AM, Locke AJ, Ali JYH, Ismail IH. Journal: STAR protocols - Sequential ChIP (ChIP-reChIP) enables the characterization of the same nucleosome for two different types of modifications or histone subtypes. Here, we describe a ChIP-reChIP protocol to identify a heterotypic (asymmetric) H2A.Z–H2A-containing
Date: (2021) Authors: Maxim Nekrasov , David J. Tremethick Ref: Enhancers and Promoters - An optimized chromatin immunoprecipitation protocol using Staph-seq for analyzing genome-wide protein-DNA interactions. new window
Date: 2022-12-09 Authors: Tao F, Rhonda E, He X, Perry JM, Li L. Journal: STAR protocols - Chromatinimmunoprecipitation (ChIP) is used to probe the presence of proteins and/or their posttranslational modifications on genomic DNA. This method is often used alongside chromosome conformation capture approaches to obtain a better-rounded vi
Date: (2021) Authors: Xue Qing Wang , Christopher J. Cameron , Dana Segal , Denis Paquette ... Josée Dostie Ref: Capturing Chromosome Conformation - Preparation of Intact Nuclei for Single-Nucleus Omics Using Frozen Cell Suspensions from Mutant Embryos of Xenopus tr... new window
Date: 2022-12-01 Authors: Nakayama T, Roubroeks JAY, Veenstra GJC, Grainger RM. Journal: Cold Spring Harbor protocols - High-Resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci and Alleles new windowThe positioning of nucleosomes regulates the accessibility of genomic DNA and can impact the activities of functional elements. Nucleosome positioning is highly consistent at each genomic location in any particular cell-type, but can vary in an
Date: (2021) Authors: Dominic van Essen , Agata Oruba , Simona Saccani Ref: Enhancers and Promoters Date: 2022-11-23 Authors: Ding Y, Liu F. Journal: STAR protocols - Proximity ligation-assisted ChIP-Seq (PLAC-Seq), also known as HiChIP, is a method to detect and quantify chromatin contacts anchored at genomic regions bound by specific proteins or histone modifications. By combining in situ Hi-C and chromatin
Date: (2021) Authors: Miao Yu , Ivan Juric , Armen Abnousi , Ming Hu , Bing Ren Ref: Enhancers and Promoters