Results 581-590 of about 900
- Genome editing with site-specific nucleases (SSNs) may be effective for gene therapy, as SSNs can modify target genes. However, the main limitation of genome editing for clinical use is off-target effects by excess amounts of SSNs within cells.
Date: (2018) Authors: Maui Nishio , Ayana Yamagishi , Kaori Tsukakoshi , Yoshio Kato ... Kazunori Ikebukuro Ref: Zinc Finger Proteins - Systems Wide Analysis of CCM Signaling Complex Alterations in CCM-Deficient Models Using Omics Approaches. new window
Date: 2020-01-01 Authors: Abou-Fadel J, Zhang J. Journal: Methods in molecular biology (Clifton, N.J.) - The ability to activate or repress specific genes in the brain could have a tremendous impact for understanding and treating neurological disorders. Artificial transcription factors based on zinc finger, TALE, and CRISPR/Cas9 programmable DNA-binding
Date: (2018) Authors: Benjamin Pyles , Barbara J. Bailus , Henriette O’Geen , David J. Segal Ref: Epigenome Editing - Two novel techniques refine genome-wide mapping of R loops.
Date: (2018) Authors: Vesna Todorovic - DNA modifications, especially methylation, are known to play a crucial part in many regulatory processes in the cell. Recently, 5-hydroxymethylcytosine (5hmC) was discovered, a DNA modification derived as an intermediate of 5-methylcytosine (5mC)
Date: (2018) Authors: Kristina Kirschner , Felix Krueger , Anthony R. Green , Tamir Chandra Ref: DNA Methylation Protocols - The diversity and huge omics data take biology and biomedicine research and application into a big data era, just like that popular in human society a decade ago. They are opening a new challenge from horizontal data ensemble (e.g., the similar types
Date: (2018) Authors: Xiang-Tian Yu , Tao Zeng Ref: Computational Systems Biology - Monitoring early S-phase origin firing and replication fork movement by sequencing nascent DNA from synchronized cells new windowA better understanding of DNA replication initiation in human cells and how this process is altered upon DNA replication stress requires the ability to study origin firing genome wide. Previously described methods of mapping DNA replication origins
Date: (2018) Authors: Morgane Macheret , Thanos D. Halazonetis Date: 2020-01-01 Authors: Günzl A, Srivastava A, Gosavi U. Journal: Methods in molecular biology (Clifton, N.J.) - Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease new windowLaser capture microdissection (LCM) is a powerful tool for selection and isolation of single cells or compartments from complex primary tissues to perform molecular analyses. Celiac disease is a genetic autoimmune disorder where the ingestion of
Date: (2018) Authors: Giuseppe Iacomino , Vera Rotondi Aufiero , Pasquale Marena , Antonella Venezia ... Giuseppe Mazzarella Ref: Laser Capture Microdissection - Integrated Chip-Seq and RNA-Seq Data Analysis Coupled with Bioinformatics Approaches to Investigate Regulatory Landscape... new window
Date: 2020-01-01 Authors: Ramos J, Felty Q, Roy D. Journal: Methods in molecular biology (Clifton, N.J.)