Results 151-160 of about 900
- Measuring occupancies of the nucleosome and nucleosome-interacting factors in vivo in Saccharomyces cerevisiae genome-wi... new window
Date: 2023-08-18 Authors: Biernat E, Khan U, Govind CK. Journal: Methods (San Diego, Calif.) - The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor. Here we describe a set of protocols for using the catalytically inactive dead Cas9 (dCas9)-based tools, including the bipartite super repressor
Date: (2023) Authors: Nan Cher Yeo , George M. Church Ref: Transcription Factor Regulatory Networks - Protocol for bulk and single-nuclei chromatin accessibility quantification in mouse liver tissue. new window
Date: 2023-08-16 Authors: Korenfeld N, Toft NI, Dam TV, Charni-Natan M, Grøntved L, Goldstein I. Journal: STAR protocols - DISCOVER-seq+ enables the efficient detection of CRISPR–Cas off-target breaks in primary cells and tissues.
Date: (2023) Authors: Carlos Jiménez , Nicola Crosetto - KLF5/MDM2 Axis Modulates Oxidative Stress and Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells: The Role... new window
Date: 2023-07-31 Authors: Li X, Chen D, Ouyang B, Wang S, Li Y, Li L, Zhu S, Zheng G. Journal: Laboratory investigation; a journal of technical methods and pathology - Bioinformatics of the Endocannabinoid System: Study of DNA Methylation at Rat Cnr1 Gene Promoter new windowIn this chapter, we will describe the bioinformatic tools that allow verifying the presence of CpG islands in a gene promoter region. We will also describe the tools needed to identify consensus motifs for specific transcription factors, focusing on
Date: (2023) Authors: Annalaura Sabatucci , Claudio D’Addario Ref: Endocannabinoid Signaling - ATAC-seq protocol for the profiling of chromatin accessibility of in situ fixed quiescent and activated muscle stem cell... new window
Date: 2023-06-22 Authors: Dong A, Chan ITC, Cheung TH. Journal: STAR protocols - Universal NicE-Seq: A Simple and Quick Method for Accessible Chromatin Detection in Fixed Cells new windowGenome-wide accessible chromatin sequencing and identification has enabled deciphering the epigenetic information encoded in chromatin, revealing accessible promoters, enhancers, nucleosome positioning, transcription factor occupancy, and other
Date: (2023) Authors: Hang Gyeong Chin , Udayakumar S. Vishnu , Zhiyi Sun , V. K. Ponnaluri ... Sriharsa Pradhan Ref: Chromatin Accessibility Date: 2023-06-05 Authors: Zhao W, Zhao J, Li K, Hu Y, Yang D, Tan B, Shi J. Journal: Laboratory investigation; a journal of technical methods and pathology - Tracking the Host Response to Infection in Peritoneal Models of Acute Resolving Inflammation new windowAntimicrobial host defense is dependent on the rapid recruitment of inflammatory cells to the site of infection, the elimination of invading pathogens, and the efficient resolution of inflammation that minimizes damage to the host. The peritoneal
Date: (2023) Authors: David Millrine , Christopher M. Rice , Javier U. Fernandez , Simon A. Jones Ref: Inflammation and Cancer