Results 191-200 of about 900
Date: 2023-01-01 Authors: Bunch H, Calderwood SK. Journal: Methods in molecular biology (Clifton, N.J.) - Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit new windowThe sequence-specific transcription factor RBPJ, also known as CSL (CBF1, Su(H), Lag1), is an evolutionarily conserved protein that mediates Notch signaling to guide cell fates. When cells enter mitosis, DNA is condensed and most transcription
Date: (2022) Authors: Kostiantyn Dreval , Robert J. Lake , Hua-Ying Fan Ref: Notch Signaling Research Date: 2023-01-01 Authors: Wang Z, Ji S, Ma JH, Chen J, Tang S. Journal: Methods in molecular biology (Clifton, N.J.) - Chromatinimmunoprecipitation (ChIP) enables the study of DNA–protein interactions. When coupled with high-throughput sequencing (ChIP-seq), this method allows the generation of genome-wide profiles of the distribution of specific proteins in a giv
Date: (2022) Authors: Julie Brind’Amour , Matthew C. Lorincz Ref: Histone Methyltransferases - The investigation of cohesin binding sites throughout different mammalian genomes by ChIP-sequencing has been fundamental to discover how cohesin and CTCF collaborate to form chromatin loops and to gain insight in the intricate regulation of cohesin.
Date: (2022) Authors: Macarena Moronta Gines , Kerstin S. Wendt Ref: Chromatin - Epigenome editing has become more precise and effective by coupling epigenetic effectors to the dCas9 protein and targeting regulatory regions such as promoters and enhancers. Here, we describe a basic methodology for performing an epigenome editing
Date: (2022) Authors: Rosa S. Guerra-Resendez , Isaac B. Hilton Ref: Riboregulator Design and Analysis Date: 2023-01-01 Authors: Andrenacci D, Cernilogar FM. Journal: Methods in molecular biology (Clifton, N.J.) - The introduction of chromosome conformation capture (3C)-based technologies coupled with next-generation sequencing have significantly advanced our understanding of how the genetic material is organized within the eukaryotic nucleus.
Date: (2022) Authors: Dafne Campigli Giammartino , Alexander Polyzos , Effie Apostolou Ref: Spatial Genome Organization - Comprehensive Survey of ChIP-Seq Datasets to Identify Candidate Iron Homeostasis Genes Regulated by Chromatin Modificati... new window
Date: 2023-01-01 Authors: Yu Y, Wang Y, Yao Z, Wang Z, Xia Z, Lee J. Journal: Methods in molecular biology (Clifton, N.J.) - Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seq new windowWe describe a protocol for H3K27ac ChIP paired-end sequencing and computational analysis of rearrangements. Our approach can be used to simultaneously map enhancers and their activity and to identify structural variations at enhancers. Since changes
Date: (2022) Authors: Israel Antman , Ester Davis , Shahd Abu-Kamel , Merav Hecht , Yotam Drier Ref: Cancer Drug Resistance