Elucidating the biological function of histone methyltransferases requires knowledge of the genomic sites at which they act. CUT&RUN represents a valuable alternative to chromatinimmunoprecipitation for the mapping of histone methylation pat

HiChIP is a novel method for the analysis of chromatin interactions based on in situ Hi-C that adds an immuno-precipitation (ChIP) step for the investigation of chromatin structures driven by specific proteins. This approach has been shown to be very

In mammalian cells, multiprotein complexes form at specific genomic regulatory elements (REs) to control gene expression, which in turn is ultimately responsible for cellular identity. Consequently, insight into the molecular composition of these

The three-dimensional structure of the genome is highly organized and is an important aspect of gene regulation. Chromatin interactions can be identified using chromosome conformation capture-based techniques, which rely on proximity ligation. Of

It has recently been demonstrated that budding yeast telomeres are transcribed into TERRA, a long noncoding RNA. Due to the G-rich nature of the coding strand, TERRA has a tendency to form DNA–RNA hybrids and potentially R-loops, which in turn,

DNA replication during S phase in eukaryotes is a highly regulated process that ensures the accurate transmission of genetic material to daughter cells during cell division. Replication follows a well-defined temporal program, which has been studied

Bisulfite sequencing is the “gold-standard” technique for DNA methylation analysis. By combining bisulfite sequencing with high-throughput, next-generation sequencing technology, we can document methylation from many thousands of individual reads