Results 211-220 of about 900
- Reprogramming the piRNA pathway for multiplexed and transgenerational gene silencing in C. elegans new windowSingle-guide RNAs can target exogenous CRISPR–Cas proteins to unique DNA locations, enabling genetic tools that are efficient, specific and scalable. Here we show that short synthetic guide Piwi-interacting RNAs (piRNAs) (21-nucleotide sg-piRNAs)
Date: (2022) Authors: Monika Priyadarshini , Julie Zhouli Ni , Amhed M. Vargas-Velazquez , Sam Guoping Gu , Christian Frøkjær-Jensen Date: 2023-01-01 Authors: Mimitou EP, Smibert P, Lareau CA. Journal: Methods in molecular biology (Clifton, N.J.) - CLIP-Seq (Deep Sequencing after in vivo Crosslinking and Immunoprecipitation, HITS-CLIP) has emerged as a key method for the study of RNA-binding proteins (RBPs), as it can scrutinize the RNAs bound by an RBP in vivo, with minimum manipulation of
Date: (2022) Authors: Abd-El Monsif Shawky , Mahmoud Dondeti , Zissimos Mourelatos , Anastasios Vourekas Ref: piRNA Date: 2023-01-01 Authors: Marinov GK, Shipony Z, Kundaje A, Greenleaf WJ. Journal: Methods in molecular biology (Clifton, N.J.) - Enzyme-free targeted DNA demethylation using CRISPR–dCas9-based steric hindrance to identify DNA methylation marks causa... new windowDNA methylation involves the enzymatic addition of a methyl group primarily to cytosine residues in DNA. This protocol describes how to produce complete and minimally confounded DNA demethylation of specific sites in the genome of cultured cells by
Date: (2022) Authors: Daniel M. Sapozhnikov , Moshe Szyf Date: 2023-01-01 Authors: Stewart-Morgan KR, Groth A. Journal: Methods in molecular biology (Clifton, N.J.) - In mammals, molecular circadian clocks not only exist in the suprachiasmatic nucleus (SCN) but in almost all organ systems. Intriguingly, tissue clocks can operate in both isolated tissues and cell lines with endocrine signals mediating the circadian
Date: (2022) Authors: Violetta Pilorz , Iwona Olejniczak , Henrik Oster Ref: Circadian Regulation Date: 2023-01-01 Authors: Sokolova M, Vartiainen MK. Journal: Methods in molecular biology (Clifton, N.J.) - Epigenomics studies require the combined analysis and integration of multiple types of data and annotations to extract biologically relevant information. In this context, sophisticated data visualization techniques are fundamental to identify
Date: (2022) Authors: Koustav Pal , Francesco Ferrari Ref: Hi-C Data Analysis - Purification of Ribosome-Nascent-Chain Complex for Ribosome Profiling and Selective Ribosome Profiling new windowSelective Ribosome Profiling (SeRP) is an emerging methodology, developed to capture cotranslational interactions in vivo. To date, SeRP is the only method that can directly capture, in near-codon resolution, ribosomes in action. Thus, SeRP allows us
Date: (2022) Authors: Hagit Bar-Yosef , Johannes Venezian , Kevin Klann , Ayala Shiber Ref: Yeast Functional Genomics