Results 221-230 of about 900
- Single-cell RNA sequencing (scRNA-seq) is emerging as an essential technique for studying the physiology of individual cells in populations. Although well-established and optimized for mammalian cells, research of microorganisms has been faced with
Date: (2022) Authors: Lieselotte Vermeersch , Abbas Jariani , Jana Helsen , Benjamin M. Heineike , Kevin J. Verstrepen Ref: Yeast Functional Genomics - Guanine-rich RNAs can fold into four-stranded structures, termed G-quadruplexes (G4-RNAs), and participate in a wide range of biological processes. Here we describe in detail a G4-RNA-specific precipitation (G4RP) protocol, which enables the
Date: (2022) Authors: Sunny Y. Yang , David Monchaud , Judy M. Wong Date: 2023-01-01 Authors: Dhillon-Richardson RM, Haugan AK, Martik ML. Journal: Methods in molecular biology (Clifton, N.J.) - Single-cell sequencing is a promising attempt to investigate the genomic, transcriptomic, and multiomic level of individual cell in the larger population of cells. The outward evolution of the technique from a manual method to the automation of
Date: (2022) Authors: Deepanshu Verma , Namyashree Nayak , Ashuthosh Singh , Ashutosh Kumar Singh , Neha Garg Ref: Medulloblastoma - The field of population genetics has exploded in the last two decades following the sequencing of the human genome in 2001 (Green et al. Nature 526:29–31, 2015). Tools to measure genetic variation have matured significantly throughout this
Date: (2022) Authors: Austin J. Asselt , Erik A. Ehli Ref: Estrogen Receptors Date: 2023-01-01 Authors: Matsui Y, Peng JC. Journal: Methods in molecular biology (Clifton, N.J.) - Single-molecule imaging is a powerful method for unveiling precise molecular mechanisms. Particularly, single-molecule analysis with total internal reflection fluorescence (TIRFTotal internal reflection fluorescence microscopy (TIRF)) microscopy has
Date: (2022) Authors: Shuting Shen , Masahiro Naganuma , Yukihide Tomari , Hisashi Tadakuma Ref: piRNA - The Nodewalk assay to quantitate chromatin fiber interactomes in very small cell populations new windowThe chromosome conformation capture method and its derivatives, such as circularized chromosome conformation capture, carbon copy chromosome conformation capture, high-throughput chromosome conformation capture and capture high-throughput chromosome
Date: (2022) Authors: Johanna Vestlund , Noriyuki Sumida , Rashid Mehmood , Deeksha Bhartiya ... Anita Göndör - CLOuD9: CRISPR-Cas9-Mediated Technique for Reversible Manipulation of Chromatin Architecture new windowThe spatial organization of the genome plays a critical role in cell-specific biological functions such as gene expression. Existing genome-wide technologies reveal a dynamic interplay between chromatin looping and gene regulation, but the mechanisms
Date: (2022) Authors: Wei Qiang Seow , Poonam Agarwal , Kevin C. Wang Ref: Spatial Genome Organization - Measuring Cytological Proximity of Chromosomal Loci to Defined Nuclear Compartments with TSA-seq new windowDistinct nuclear structures and bodies are involved in genome intranuclear positioning. Measuring proximity and relative distances of genomic loci to these nuclear compartments, and correlating this chromosome intranuclear positioning with epigenetic
Date: (2022) Authors: Liguo Zhang , Yu Chen , Andrew S. Belmont Ref: Spatial Genome Organization