Results 31-40 of about 900
Date: 2025-01-01 Authors: De Kumar B, Krishnan J. Journal: Methods in molecular biology (Clifton, N.J.) - DNA-protein interactions play fundamental roles in diverse biological functions. The gene-centered method is used to identify the upstream regulators of defined genes. In this study, we developed a novel method for capturing the proteins that bind to
Date: (2024) Authors: Xuejing Wen , Yucheng Wang Ref: ChromatinImmunoprecipitation - ChromatinImmunoprecipitation in Adipose Tissue and Adipocytes: How to Proceed and Optimize the Protocol for Transcriptio... new windowChromatinimmunoprecipitation (ChIP) coupled to qPCR or sequencing is a crucial experiment to determine direct transcriptional regulation under the control of specific transcriptional factors or co-regulators at loci-specific or pan-genomic level
Date: (2024) Authors: Céline Jouffe , Kenneth A. Dyar , Nina Henriette Uhlenhaut Ref: ChromatinImmunoprecipitation Date: 2025-01-01 Authors: Saotome M, Goodman J, Takaku M. Journal: Methods in molecular biology (Clifton, N.J.) - Chromatinimmunoprecipitation (ChIP) analysis followed by genome-wide sequencing represents the most efficient method for studying the complex interactions between nuclear proteins and their associated genomic DNA. This chapter aims to present
Date: (2024) Authors: Fahd Alhamdan Ref: Food Allergens - Multiplexed chromatinimmunoprecipitation sequencing for quantitative study of histone modifications and chromatin factors new windowChIP–seq is a widely used technique for studying histone post-translational modifications and DNA-binding proteins. DNA fragments associated with a specific protein or histone modification epitope are captured by using antibodies, sequenced and
Date: (2024) Authors: Banushree Kumar , Carmen Navarro , Philip Yuk Yung , Jing Lyu ... Simon J. Elsässer Date: 2025-01-01 Authors: Fu Y, Simonini S. Journal: Methods in molecular biology (Clifton, N.J.) - Chromatinimmunoprecipitation (ChIP) is used to analyze the targeting of a protein to a specific region of chromatin in vivo. Here, we present an instructive ChIP protocol for Arabidopsis imbibed seeds. The protocol covers all steps, from t
Date: (2024) Authors: Yongmin Kwon , Giltsu Choi Ref: Seed Dormancy - ATAC-seq for Characterizing Host and Pathogen Genome Accessibility During Virus Infection. new window
Date: 2025-01-01 Authors: Liu D, Howard TR, Cristea IM. Journal: Methods in molecular biology (Clifton, N.J.) - ChromatinImmunoprecipitation to Investigate H2A.Z Dynamics in Response to Environmental Changes new windowDNA methylation and posttranslational modifications of histones instruct gene expression in eukaryotes. Besides canonical histones, histone variants also play a critical role in transcriptional regulation. One of the best studied histone variants in
Date: (2024) Authors: J. Paola Guzmán , Mark Zander , Björn C. Willige Ref: Thermomorphogenesis