Results 331-340 of about 900
- Chromatinimmunoprecipitation coupled with sequencing (ChIP-seq) is a widely used method for mapping the genome-wide locations of chromatin-associated proteins. This protocol has been developed and utilized to perform ChIP on histone covale
Date: (2020) Authors: William A. Ricci , Laura Levin , Xiaoyu Zhang Ref: Cereal Genomics Date: 2022-01-01 Authors: Salehin N, Santucci N, Osteil P, Tam PPL. Journal: Methods in molecular biology (Clifton, N.J.) - Chromatinimmunoprecipitation followed by next-generation DNA sequencing (ChIP-seq) has been used to identify transcription factor (TF) binding proteins throughout the genome. Unfortunately, this approach traditionally requires commercial
Date: (2020) Authors: Sarah K. Meadows , Laurel A. Brandsmeier , Kimberly M. Newberry , Michael J. Betti ... Richard M. Myers Ref: Stem Cell Transcriptional Networks Date: 2022-01-01 Authors: Gehrke AR, Srivastava M. Journal: Methods in molecular biology (Clifton, N.J.) - Chromatin organization and epigenetic marks play a critical role in stem cell pluripotency and differentiation. Chromatin digestion by micrococcal nuclease (MNase) followed by high-throughput sequencing (MNase-seq) is the most widely used genome-wide
Date: (2020) Authors: Paula Beati , Răzvan V. Chereji Ref: Stem Cell Transcriptional Networks - Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres new windowCharacterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence
Date: (2020) Authors: Šárka Schořová , Jiří Fajkus , Petra Procházková Schrumpfová Ref: The Nucleus - DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatinimmunoprecipitation (ChIP) experiments followed
Date: (2020) Authors: Ronaldo Carvalho Augusto , David Roquis , Marion Picard , Cristian Chaparro ... Christoph Grunau Ref: Schistosoma mansoni - FiTAc-seq: fixed-tissue ChIP-seq for H3K27ac profiling and super-enhancer analysis of FFPE tissues new windowFixed-tissue ChIP-seq for H3K27 acetylation (H3K27ac) profiling (FiTAc-seq) is an epigenetic method for profiling active enhancers and promoters in formalin-fixed, paraffin-embedded (FFPE) tissues. We previously developed a modified ChIP-seq protocol
Date: (2020) Authors: Alba Font-Tello , Nikolas Kesten , Yingtian Xie , Len Taing ... Henry W. Long - Single-Cell Transcriptomic and Epigenetic Analyses of Mouse Mammary Development Starting with the Embryo. new window
Date: 2022-01-01 Authors: Ma Z, Lytle NK, Ramos C, Naeem RF, Wahl GM. Journal: Methods in molecular biology (Clifton, N.J.) - Measurement of differential chromatin interactions with absolute quantification of architecture (AQuA-HiChIP) new windowMethods developed to capture protein-anchored chromatin interactions (chromatin interaction analysis by paired-end tag sequencing and HiChIP) have yielded tremendous insights into the 3D folding principles of the genome, but are normalized by
Date: (2020) Authors: Berkley E. Gryder , Javed Khan , Benjamin Z. Stanton