Results 341-350 of about 900
Date: 2022-01-01 Authors: Sahadevan S, Pérez-Berlanga M, Polymenidou M. Journal: Methods in molecular biology (Clifton, N.J.) - Formation of DNA:RNA hybrids or R-loopsR-loops contributes to numerous biologic processes. The development of the S9.6 antibody makes the analysis of R-LoopsR-loops (DNA:RNA hybrids) possible through immunoprecipitation. Here, we describe the
Date: (2020) Authors: Hunter R. Gibbons , Thomas M. Aune Ref: RNA-Chromatin Interactions - Simultaneous profiling of chromatin accessibility and methylation on human cell lines with nanopore sequencing new windowProbing epigenetic features on DNA has tremendous potential to advance our understanding of the phased epigenome. In this study, we use nanopore sequencing to evaluate CpG methylation and chromatin accessibility simultaneously on long strands of DNA
Date: (2020) Authors: Isac Lee , Roham Razaghi , Timothy Gilpatrick , Michael Molnar ... Winston Timp - Plant metabolic gene clusters consist of neighboring genes that are involved in the biosynthesis of secondary or specialized metabolites. The genes within clusters are typically co-regulated, share a common set of chromatin marks, and code for the
Date: (2020) Authors: Ancheng C. Huang , Hans-Wilhelm Nützmann Ref: Plant Epigenetics and Epigenomics - Single-Molecule Multikilobase-Scale Profiling of Chromatin Accessibility Using m6A-SMAC-Seq and m6A-CpG-GpC-SMAC-Seq. new window
Date: 2022-01-01 Authors: Marinov GK, Shipony Z, Kundaje A, Greenleaf WJ. Journal: Methods in molecular biology (Clifton, N.J.) - Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions new windowMapping the binding sites of DNA- or chromatin-interacting proteins is essential to understand many essential biological processes. Methyl Adenine Identification (MadID) is a proximity methylation-based assay that allows the visualization,
Date: (2020) Authors: David Umlauf , Michal Sobecki , Laure Crabbe Ref: The Nucleus Date: 2022-01-01 Authors: Cejas P, Long HW. Journal: Methods in molecular biology (Clifton, N.J.) - DISCOVER-seq (discovery of in situ Cas off-targets and verification by sequencing) is a broadly applicable approach for unbiased CRISPR–Cas off-target identification in cells and tissues. It leverages the recruitment of DNA repair factors to
Date: (2020) Authors: Beeke Wienert , Stacia K. Wyman , Charles D. Yeh , Bruce R. Conklin , Jacob E. Corn - Characterization of R-Loop Structures Using Single-Molecule R-Loop Footprinting and Sequencing new windowR-loops are three-stranded structures that form during transcription when the nascent RNA hybridizes with the template DNA resulting in a DNA:RNA hybrid and a looped-out single-stranded DNA (ssDNA) strand. These structures are important for normal
Date: (2020) Authors: Maika Malig , Frederic Chedin Ref: RNA-Chromatin Interactions - Eukaryotic gene transcription is associated with the eviction of nucleosomes and the formation of open chromatin, which enables the recruitment of transcriptional coactivators and other regulatory factors. Open chromatin is thus a hallmark of
Date: (2020) Authors: Stephani Baum , Eva-Maria Reimer-Michalski , Michal R. Jaskiewicz , Uwe Conrath