Results 361-370 of about 900
- ChromatinImmunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory new windowChromatinimmunoprecipitation assays permit the isolation and subsequent identification of genomic DNA (gDNA) fragments bound directly or indirectly to proteins of interest, including transcription factors, co-factors, or chromatin remodeli
Date: (2019) Authors: Hunter McColl , Jamie L. Zagozewski , David D. Eisenstat Ref: Brain Tumor Stem Cells - Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatinimmunoprecipitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extrac
Date: (2019) Authors: Kelly B. Wolfe , David T. Long Ref: DNA Repair - lncENST Suppress the Warburg Effect Regulating the Tumor Progress by the Nkx2-5/ErbB2 Axis in Hepatocellular Carcinoma. new window
Date: 2021-12-06 Authors: Chen G, Jiang J, Wang X, Feng K, Ma K. Journal: Computational and mathematical methods in medicine - ChromatinImmunoprecipitation Assay for Analyzing Transcription Factor Activity at the Level of Peripheral Myelin Gene Pr... new windowDisruption of epigenetic regulators of transcription is a central mechanism of oncogenesis. Differential gene expression is facilitated by transcriptional regulatory mechanisms and chromatin modifications through DNA-protein interactions. One of the
Date: (2019) Authors: Joelle Makoukji Ref: Psychiatric Disorders - Research on Radiosensitivity of the Protein Kinase B Signaling Pathway in Cervical Cancer. new window
Date: 2021-11-23 Authors: Zhu Y, Xu L, Ma W, Chen Z. Journal: Computational and mathematical methods in medicine - ChromatinImmunoprecipitation Assay to Analyze the Effect of G-Quadruplex Interactive Agents on the Binding of RNA Polyme... new windowGrowing evidence suggests the existence of G-quadruplexes and their involvement in transcriptional regulation of many human genes, including VEGF. These studies also provide strong evidence that G-quadruplex structures are stabilized by binding to
Date: (2019) Authors: Daekyu Sun Ref: G-Quadruplex Nucleic Acids - A proteomics protocol to identify stimulation-induced binding partners dependent on a specific gene in mammalian cells. new window
Date: 2021-11-13 Authors: Zhu J, Xiao W. Journal: STAR protocols - Chromatinimmunoprecipitation (ChIP) in conjunction with qPCR or next generation sequencing (ChIP-seq) is used to detect protein–DNA interaction. Typically, DNA bound to a protein of interest is captured with an antibody against this protein, and D
Date: (2019) Authors: Victoria Adelheid Malik , Barbara Benedetto , Mira Jakovcevski Ref: Astrocytes - Genomic mapping of DNA-repair reaction intermediates in living cells with engineered DNA structure-trap proteins. new window
Date: 2021-10-29 Authors: Liu J, Mei Q, Nimer S, Fitzgerald DM, Rosenberg SM. Journal: Methods in enzymology - Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by ChromatinImmunoprecipitation... new windowChromatinimmunoprecipitation (ChIP) is widely used to measure protein-DNA interactions. This protocol outlines a ChIP method used to identify the association of a protein or protein modification (such as a specific histone modification—methylation
Date: (2019) Authors: Shama Bhatia , Jason Matthews , Peter G. Wells Ref: Developmental Toxicology