Results 381-390 of about 900
Date: 2021-10-11 Authors: Chavkin NW, Cain S, Walsh K, Hirschi KK. Journal: Journal of visualized experiments : JoVE - Native internally calibrated chromatinimmunoprecipitation for quantitative studies of histone post-translational modific... new windowChromatinimmunoprecipitation coupled to next-generation sequencing (ChIP-seq) has served as the central method for the study of histone modifications for the past decade. In ChIP-seq analyses, antibodies selectively capture nucleosomes bearing
Date: (2019) Authors: Adrian T. Grzybowski , Rohan N. Shah , William F. Richter , Alexander J. Ruthenburg - An optimized ATAC-seq protocol for genome-wide mapping of active regulatory elements in primary mouse cortical neurons. new window
Date: 2021-09-30 Authors: Maor-Nof M, Shipony Z, Marinov GK, Greenleaf WJ, Gitler AD. Journal: STAR protocols - Epi-ID: Systematic and Direct Screening for Chromatin Regulators in Yeast by Barcode-ChIP-Seq new windowThe assembly and regulation of chromatin requires coordinated activity of multiple mechanisms. Many factors feed into signaling networks that control the epigenome of a cell. It is this complexity that makes understanding the layers of epigenetic
Date: (2019) Authors: Deepani W. Poramba-Liyanage , Tessy Korthout , Fred van Leeuwen Ref: Yeast Systems Biology - Chromatinimmunoprecipitation (ChIP) coupled to quantitative real-time PCR (ChIP-qPCR) or Next-Generation Sequencing (ChIP-seq) enables us to study the dynamics of chromatin recruitment of transcription factors (TFs). The popular model syst
Date: (2019) Authors: Neeraj Kumar , Arnab Mukhopadhyay Ref: FOXO Transcription Factors - Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast. new window
Date: 2021-09-14 Authors: Challa K, Seebacher J, Gasser SM. Journal: STAR protocols - Nascent RNA may form a three-stranded structure with DNA, called an R-loop, which has been linked to fundamental biological processes such as transcription, replication and genome instability. Here, we provide a detailed protocol for a newly
Date: (2019) Authors: Jia-Yu Chen , Xuan Zhang , Xiang-Dong Fu , Liang Chen - Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing. new window
Date: 2021-09-08 Authors: Loft A, Herzig S, Schmidt SF. Journal: STAR protocols - Analysis of Interaction Between Long Noncoding RNAs and Protein by RNA Immunoprecipitation in Arabidopsis new windowLong noncoding RNAs (lncRNAs) play important roles in several processes including control of gene expression. These RNAs function through binding to histone-modifying complexes and transcriptional machinery including transcription factor, mediator,
Date: (2019) Authors: Jun Sung Seo , Nam-Hai Chua Ref: Plant Long Non-Coding RNAs - Optimized protocols for ChIP-seq and deletion mutant construction in Pseudomonas syringae . new window
Date: 2021-08-25 Authors: Yao C, Shao X, Li J, Deng X. Journal: STAR protocols