Results 591-600 of about 900
- In Vitro Approaches to Identify Thyroid Hormone Receptor-Dependent Transcriptional Response new windowThe thyroid hormones act through their nuclear receptor TRs that are T3-modulated transcription factors. To elucidate the molecular mechanisms at the basis of specific physiological responses to TH-TR, in vitro approaches are commonly used to
Date: (2018) Authors: Maria Sirakov , Michelina Plateroti Ref: Thyroid Hormone Nuclear Receptor - Studying Transcription Factor Binding to Specific Genomic Loci by ChromatinImmunoprecipitation (ChIP) new windowPlant hormone signaling involves complex transcriptional networks, where transcription factors orchestrate the control of specific gene expression. These networks include cross talk between hormone signaling pathways, and the integration of
Date: (2017) Authors: S. Vinod Kumar , Doris Lucyshyn Ref: Plant Hormones Date: 2020-01-01 Authors: Beati P, Chereji RV. Journal: Methods in molecular biology (Clifton, N.J.) - Chromatinimmunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is widely used for studying the nucleoprotein components that are involved in the various cellular processes required for shaping the bacterial nucleoid. Th
Date: (2017) Authors: Roxanne E. Diaz , Aurore Sanchez , Véronique Anton Le Berre , Jean-Yves Bouet Ref: The Bacterial Nucleoid Date: 2020-01-01 Authors: Lerdrup M, Hansen K. Journal: Methods in molecular biology (Clifton, N.J.) - Telomere chromatinimmunoprecipitation (ChIP) is an experimental method used to determine whether proteins are associated with telomere DNA inside the nuclei of cells and tissues. Telomere-associated proteins are first covalently crosslinked
Date: (2017) Authors: Feng Liu , Xuyang Feng , Wenbin Ma Ref: Telomeres and Telomerase - Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using C... new windowTranscriptional activation by STAT5 is repressed by deacetylase inhibitors. Investigating the role of deacetylases (HDACs) in STAT5-mediated transcription implies the analysis of molecular events taking place at the chromatin level. We describe here
Date: (2017) Authors: Sophia Pinz , Anne Rascle Ref: HDAC/HAT Function Assessment and Inhibitor Development Date: 2020-01-01 Authors: Fomin V, Prives C. Journal: Methods in molecular biology (Clifton, N.J.) - Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by ChromatinImmunoprecipitation Assays new windowChromatinimmunoprecipitation (ChIP) is a valuable technique for localizing proteins of interest to specific genomic sites and determining the relative abundance of these proteins at these sites. The ChIP method entails chemical cross-linking
Date: (2017) Authors: Julie Huang , Nahid Iglesias , Danesh Moazed Ref: The Mitotic Exit Network - Characterization of Cell-Type-Specific DNA Binding Sites of Plant Transcription Factors Using ChromatinImmunoprecipitati... new windowThe generation of diverse cell types in multicellular organisms often requires the activity of cell-type-specific transcription factors. Understanding where these transcription factors bind in controlling specific cellular programs is critical.
Date: (2017) Authors: On Sun Lau Ref: Plant Gene Regulatory Networks